Flow Cytometry Laboratory (Flow Lab)֍

The is a 3D picture that shows the Flow Lab. The image was taken from a 3D dollhouse projection where the user can virtually "walk" through the Flow Lab.

Click on the picture above to take a virtual tour of the Flow Lab.

Contact

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Mission

The mission of the UWCCC Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology and treatment in basic, translational and clinical research projects.

Rigor and Reproducibility

  • R&R Statement
    Word        PDF

Letter of Support

  • Letter of Support Template
    Word        PDF

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Publications

  1. Back JB, Martinez L, Nettenstrom L, Sheerar D, Thornton S. Establishing a biosafety plan for a flow cytometry shared resource laboratory. Cytometry A. 2022 May;101(5):380-386. doi: 10.1002/cyto.a.24524. Epub 2022 Jan 17. PMID: 35037390; PMCID: PMC9081124.
  2. Carlson PM, Mohan M, Patel RB, Birstler J, Nettenstrom L, Sheerar D, Fox K, Rodriguez M, Hoefges A, Hernandez R, Zahm C, Kim K, McNeel DG, Weichert J, Morris ZS, Sondel PM. Optimizing Flow Cytometric Analysis of Immune Cells in Samples Requiring Cryopreservation from Tumor-Bearing Mice. J Immunol. 2021 Jul 15;207(2):720-734. doi: 10.4049/jimmunol.2000656. Epub 2021 Jul 14. PMID: 34261667; PMCID: PMC8313010.
  3. Vazquez J, Sheerar D, Stanic AK, Patankar MS. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer. Methods Mol Biol. 2022;2424:43-58. doi: 10.1007/978-1-0716-1956-8_3. PMID: 34918286.

֍ This core facility utilizes iLab, an online management tool that allows researchers to request services, schedule equipment and manage billing with UW shared resources.