SMPH Shared Resources
School of Medicine and Public Health

Genome Editing and Animal Models Shared Resource (GEAM)

This is an accordion element with a series of buttons that open and close related content panels.

Mission

The Genome Editing and Animal Models Shared Resource provides affordable genome editing resources to the members of the members of SMPH. GEAM provides genome editing design, generation of novel models, cryopreservation, recovery and rederivation of unique mouse and rat lines. It uses the personnel, facilities and equipment of the campus-wide University of Wisconsin Biotechnology Center.

Contact

  • Phone: (608) 262-9495

Facilities

Rigor and Reproducibility

  • R&R Statement
    Word        PDF

Letter of Support

  • Letter of Support Template
    Word        PDF

This is an accordion element with a series of buttons that open and close related content panels.

Publications

  1. Foster HR, Ho T, Potapenko E, Sdao SM, Huang SM, Lewandowski SL, VanDeusen HR, Davidson SM, Cardone RL, Prentki M, Kibbey RG, Merrins MJ. β-cell deletion of the PKm1 and PKm2 isoforms of pyruvate kinase in mice reveals their essential role as nutrient sensors for the KATP channel. Elife. 2022 Aug 23;11:e79422. doi: 10.7554/eLife.79422. PMID: 35997256; PMCID: PMC9444242.
  2.  Wilson RH, Carney PR, Glover E, Parrott JC, Rojas BL, Moran SM, Yee JS, Nukaya M, Goetz NA, Rubinstein CD, Krentz KJ, Xing Y, Bradfield CA. Generation of an Allelic Series at the Ahr Locus Using an Edited Recombinant Approach. Toxicol Sci. 2021 Apr 12;180(2):239-251. doi: 10.1093/toxsci/kfab005. PMID: 33480436; PMCID: PMC8041461.
  3. Rubinstein CD, McLean DT, Lehman BP, Meudt JJ, Schomberg DT, Krentz KJ, Reichert JL, Meyer MB, Adams M, Konsitzke CM, Shanmuganayagam D. Assessment of Mosaicism and Detection of Cryptic Alleles in CRISPR/Cas9-Engineered Neurofibromatosis Type 1 and TP53 Mutant Porcine Models Reveals Overlooked Challenges in Precision Modeling of Human Diseases. Front Genet. 2021 Sep 23;12:721045. doi: 10.3389/fgene.2021.721045. PMID: 34630515; PMCID: PMC8495252.
  4. Banerjee D, Langberg K, Abbas S, Odermatt E, Yerramothu P, Volaric M, Reidenbach MA, Krentz KJ, Rubinstein CD, Brautigan DL, Abbas T, Gelfand BD, Ambati J, Kerur N. A non-canonical, interferon-independent signaling activity of cGAMP triggers DNA damage response signaling. Nat Commun. 2021 Oct 27;12(1):6207. doi: 10.1038/s41467-021-26240-9. PMID: 34707113; PMCID: PMC8551335.
  5. Peotter JL, Pustova I, Lettman MM, Shatadal S, Bradberry MM, Winter-Reed AD, Charan M, Sharkey EE, Alvin JR, Bren AM, Oie AK, Chapman ER, Salamat MS, Audhya A. TFG regulates secretory and endosomal sorting pathways in neurons to promote their activity and maintenance. Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2210649119. doi: 10.1073/pnas.2210649119. PMID: 36161950; PMCID: PMC9546632.